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Overexpression of low‐density lipoprotein receptor and lipid accumulation in intestinal polyps in Min mice
Author(s) -
Mutoh Michihiro,
Komiya Masami,
Teraoka Naoya,
Ueno Toshiya,
Takahashi Mami,
Kitahashi Tsukasa,
Sugimura Takashi,
Wakabayashi Keiji
Publication year - 2009
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.24667
Subject(s) - lipid droplet , oil red o , intestinal polyp , ldl receptor , medicine , lipid metabolism , endocrinology , triglyceride , lipoprotein , chemistry , small intestine , lipoprotein lipase , biology , cholesterol , adipose tissue , adipogenesis
Apc ‐deficient Min mice feature low expression of lipoprotein lipase (LPL), high concentration of serum triglyceride (TG), fatty change of the liver and large numbers of intestinal polyps. We have reported that induction of LPL expression reduces serum lipid, especially TG, improves fatty change of the liver and inhibits intestinal polyp formation in the mice. In this study, fatty change/lipid accumulation in intestinal mucosa and polyps in Min mice were analyzed by Oil‐red O staining and electron microscopy. A number of large lipid droplets were found in the epithelia of the upper part of polyps. On the other hand, small lipid droplets were only slightly observed at the tip of the villi in non‐tumoros parts of the small intestine of Min mice and in the villi of wild‐type mice. Moreover, low‐density lipoprotein receptor (LDLR) was overexpressed in the area where lipid droplets were observed. The expression levels of LDLR mRNA in the intestinal polyps of Min mice were ∼3 times higher compared to those in the non‐tumoros parts. Remarkable expression of cyclooxygenase‐2 was mainly distributed in stromal cells and some in epithelial cells. It is speculated that lipid accumulation in the intestinal polyps may play an important role in intestinal polyp formation in Apc ‐deficient mice. © 2009 UICC