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Upregulation of caveolin‐1 and CD147 expression in nasopharyngeal carcinoma enhanced tumor cell migration and correlated with poor prognosis of the patients
Author(s) -
Du ZiMing,
Hu ChunFang,
Shao Qiong,
Huang MaYan,
Kou ChangWei,
Zhu XiaoFeng,
Zeng YiXin,
Shao JianYong
Publication year - 2009
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.24531
Subject(s) - downregulation and upregulation , gene silencing , nasopharyngeal carcinoma , cancer research , matrix metalloproteinase , caveolin 1 , metastasis , secretion , cell migration , medicine , cell , cancer , biology , radiation therapy , gene , biochemistry , genetics
Expression of caveolin‐1 (Cav‐1) and extracellular matrix metalloproteinase inducer (EMMPRIN/CD147) and their prognostic significance were analyzed in archive NPC samples. Cav‐1 and CD147 were overexpressed in 49.48% (96/194) and 59.39% (117/197) of NPC, respectively. Both Cav‐1 and CD147 expression levels correlated significantly with metastasis ( p = 0.025 and 0.017, respectively) and a lower 5‐year survival rate ( p = 0.02 and 0.0009, respectively). In addition, Cav‐1 expression levels correlated significantly with local recurrence ( p = 0.038). Multivariate Cox regression analysis indicated that combination of high Cav‐1 and CD147 expression was a significant, independent prognosis predictor in patients with NPC (HR = 2.135; p = 0.006). Functional studies revealed that overexpression of Cav‐1 promoted secretion of MMP‐3 and MMP‐11 (active) proteins, as well as an increase in the migratory ability of CNE1 and CNE2 cells, while siRNA‐mediated silencing of Cav‐1 or CD147 led to reduced levels of MMP‐3 and MMP‐11(active) secretion, and reduced migration capacity of CNE1 and CNE2 cells. We observed a positive correlation between Cav‐1 and CD147 expression in NPC (ρ = 0.330, p = 0.000), CD147 protein levels were upregulated in Cav‐1 overexpressing CNE1 and CNE2 cells, whereas siRNA‐mediated silencing of Cav‐1 led to the downregulation of CD147 expression. Our results indicate that Cav‐1 and CD147 overexpression predict poor NPC prognosis and enhanced tumor cell migration, which is associated with MMP‐3 and MMP‐11 (active) secretion. © 2009 UICC