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JAM‐A expression positively correlates with poor prognosis in breast cancer patients
Author(s) -
McSherry Elaine A.,
McGee Sharon F.,
Jirstrom Karin,
Doyle Emma M.,
Brennan Donal J.,
Landberg Goran,
Dervan Peter A.,
Hopkins Ann M.,
Gallagher William M.
Publication year - 2009
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.24498
Subject(s) - gene knockdown , breast cancer , cancer research , integrin , tissue microarray , cell migration , biology , metastasis , microarray analysis techniques , cancer , cancer cell , cell adhesion , downregulation and upregulation , cell adhesion molecule , small hairpin rna , cell , microbiology and biotechnology , medicine , gene expression , cell culture , gene , genetics
The cell–cell adhesion protein junctional adhesion molecule‐A (JAM‐A) influences epithelial cell morphology and migration. As migration is required for tumor cell invasion and metastasis, we sought to elucidate the role of JAM‐A in invasive breast cancer. A breast cancer tissue microarray was analyzed for JAM‐A protein expression, in parallel with analysis of JAM‐A gene expression data from a breast cancer clinical dataset. Our data demonstrate a novel association between JAM‐A gene and protein upregulation and poor prognosis in breast cancer. To mechanistically dissect this process, we used lentiviral technology to stably knock down JAM‐A gene expression by shRNA in MCF7 breast cancer cells, which express high‐endogenous levels of JAM‐A. We also antagonized JAM‐A function in wild‐type MCF7 cells using an inhibitory antibody that blocks JAM‐A dimerization. Knockdown or functional antagonism of JAM‐A decreased breast cancer cell migration in scratch‐wound assays. Reductions in β1‐integrin protein levels were observed after JAM‐A‐knockdown in MCF7 cells, suggesting a mechanism for reduced motility after loss of JAM‐A. Consistent with this hypothesis, tissue microarray analysis of β1‐integrin protein expression in invasive breast cancer tissues revealed a trend toward high β1‐integrin protein levels being indicative of poor prognosis. Twenty‐two percent of patients were observed to coexpress high levels of JAM‐A and β1‐integrin protein, and MDA‐MB‐231 breast cells stably overexpressing JAM‐A showed an increase in β1‐integrin protein expression. Our results are consistent with a previously unreported role for JAM‐A overexpression as a possible mechanism contributing to progression in primary breast cancer; and a potential therapeutic target. © 2009 UICC