Premium
Global and gene‐specific promoter methylation changes are related to anti ‐B[ a ]PDE‐DNA adduct levels and influence micronuclei levels in polycyclic aromatic hydrocarbon‐exposed individuals
Author(s) -
Pavanello Sofia,
Bollati Valentina,
Pesatori Angela Cecilia,
Kapka Lucyna,
Bolognesi Claudia,
Bertazzi Pier Alberto,
Baccarelli Andrea
Publication year - 2009
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.24492
Subject(s) - methylation , dna methylation , micronucleus test , microbiology and biotechnology , benzo(a)pyrene , dna adduct , pyrene , dna , promoter , chemistry , polycyclic aromatic hydrocarbon , dna damage , gene , biology , gene expression , biochemistry , toxicity , environmental chemistry , organic chemistry
We investigated the effect of chronic exposure to polycyclic aromatic hydrocarbons (PAHs) on DNA methylation states (percentage of methylated cytosines (%mC)) in Polish male nonsmoking coke‐oven workers and matched controls. Methylation states of gene‐specific promoters ( p53 , p16 , HIC1 and IL‐6 ) and of Alu and LINE‐1 repetitive elements, as surrogate measures of global methylation, were quantified by pyrosequencing in peripheral blood lymphocytes (PBLs). DNA methylation was evaluated in relation to PAH exposure, assessed by urinary 1‐pyrenol and anti‐benzo[ a ]pyrene diolepoxide ( anti ‐B[ a ]PDE)‐DNA adduct levels, a critical genetic damage from B[ a ]P. We also evaluated whether PAH‐induced DNA methylation states were in turn associated with micronuclei in PBLs, an indicator of chromosomal instability. © 2009 UICC