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Vaccination with xenogeneic tumor endothelial proteins isolated in situ inhibits tumor angiogenesis and spontaneous metastasis
Author(s) -
Zhang Wang,
Liu JianNing,
Tan XiangYang
Publication year - 2009
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.24362
Subject(s) - angiogenesis , metastasis , cancer research , immunotherapy , adoptive cell transfer , primary tumor , angiostatin , immunology , in vivo , endothelial stem cell , medicine , pathology , biology , cancer , immune system , in vitro , t cell , biochemistry , microbiology and biotechnology
Angiogenesis is critical for tumor growth and metastasis. Tumor tissues induce the expression of angiogenesis‐associated proteins on endothelial surface that can be targeted for tumor immunotherapy. In our study, the rat tumor endothelial proteins (EP) were isolated in situ via biotinylation of tumor vascular endothelial luminal surface followed by streptavidin affinity chromatography. The isolated tumor EP contained numerous up‐regulated angiogenesis‐associated endothelial proteins. The administration of these tumor EP as a vaccine to mice reduced the microvessel density in subcutaneous primary LLC tumors, delayed spontaneous LLC tumor metastasis and prolonged post‐surgery life span. T lymphocytes from tumor EP‐vaccinated mice lysed human umbilical vascular endothelial cells, but not tumor cells in vitro , in a dose‐dependent manner. Furthermore, adoptive transfer of antitumor EP antibodies in vivo targeted to tumor endothelium and inhibited spontaneous LLC tumor metastasis. This study provides a successful preclinical exploration of the active immunotherapy for tumor by targeting tumor angiogenesis. © 2009 UICC