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DNA repair phenotype and cancer susceptibility—A mini review
Author(s) -
Li Chunying,
Wang LiE.,
Wei Qingyi
Publication year - 2008
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.24126
Subject(s) - dna repair , nucleotide excision repair , biology , dna damage , carcinogen , dna , benzo(a)pyrene , microbiology and biotechnology , xeroderma pigmentosum , mutagen , genetics , cancer research
DNA repair is a complicated biological process, consisting of several distinct pathways, that plays a fundamental role in the maintenance of genomic integrity. The very important field of DNA repair and cancer risk has developed rapidly in the past decades. In this review of selected published data from our laboratory, we describe mostly our work on the study of phenotypic markers of nucleotide excision repair (NER), as measured by the benzo(a)pyrene diol epoxide (BPDE)/ultraviolet (UV)‐induced mutagen sensitivity assays, BPDE‐induced adduct assay, host cell reactivation (HCR)‐DNA repair capacity (DRC) assay, reverse transcription‐polymerase chain reaction (RT‐PCR) assay and reverse‐phase protein lysate microarray (RPP) assay, by using peripheral blood lymphocytes in a series of molecular epidemiological studies. Results of our studies suggest that individuals with reduced DRC have an elevated cancer risk. This finding needs additional validation by other investigators, and we also discussed issues in conducting this kind of research in the future. © 2008 Wiley‐Liss, Inc.