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PKC alpha protein but not kinase activity is critical for glioma cell proliferation and survival
Author(s) -
Cameron Angus J.,
Procyk Katarzyna J.,
Leitges Michael,
Parker Peter J.
Publication year - 2008
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.23560
Subject(s) - protein kinase c , cell growth , glioma , cancer research , protein kinase a , alpha (finance) , cell survival , biology , kinase , microbiology and biotechnology , medicine , cell culture , biochemistry , genetics , surgery , construct validity , patient satisfaction
Protein kinase C alpha (PKCα) has been implicated in tumor development with high levels of PKCα expression being associated with various malignancies including glioblastomas and tumors of the breast and prostate. To account for its upregulation in these cancers, studies have suggested that PKCα plays a role in promoting cell survival. Here we show by siRNA depletion in U87MG glioma cells that a critical threshold level of PKCα protein expression is essential for their growth in the presence of serum and for their survival following serum deprivation. Derivation of PKCα wt and KO mouse embryo fibroblast cell lines confirms a role for PKCα in protecting cells from apoptosis induced by serum deprivation. Notably, PKCα was found to mediate chemo‐protection in these fibroblastic cell lines. In U87MG cells PKCα does not confer chemoprotection though this likely reflects growth arrest associated with its depletion. To determine the requirements for catalytic function, comparison was made between distinct classes of PKC inhibitors. In contrast to loss of PKCα protein, inhibition of PKC kinase activity in glioma cell lines does not significantly inhibit growth or survival. Conversely, inhibition with calphostin C, which targets the regulatory domain of PKC, potently inhibits proliferation and induces apoptosis. Evidence is presented that it is the fully phosphorylated, folded form of PKCα that confers this activity‐independent behaviour. These results indicate an essential pro‐proliferative and pro‐survival role for PKCα in glioma but question the use of ATP competitive inhibitors as therapeutics, either alone, or in combination with chemotoxic agents. © 2008 Wiley‐Liss, Inc.