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Expression and enzyme activity of α(1,6)fucosyltransferase in human colorectal cancer
Author(s) -
MuineloRomay Laura,
VázquezMartín Cristina,
VillarPortela Susana,
Cuevas Elisa,
GilMartín Emilio,
FernándezBriera Almudena
Publication year - 2008
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.23521
Subject(s) - colorectal cancer , immunohistochemistry , enzyme assay , enzyme , western blot , infiltration (hvac) , pathology , fucosyltransferase , biology , lymph node , cancer research , cancer , medicine , gene , biochemistry , physics , thermodynamics
Changes in enzyme activity and the expression levels of α(1,6)fucosyltransferase [α(1,6)FT] have been reported in certain types of malignant transformations. To develop a better understanding of the role of α(1,6)FT in human colorectal carcinoma (CRC), we analysed the enzyme activity in healthy and tumour tissues. α(1,6)FT activity was considerably higher in tumour tissue than in healthy tissue and was related to gender, lymph node metastasis, type of growth and tumour stage. We also observed a significant increase in the α(1,6)FT expression in tumour tissues as compared to healthy and transitional tissues, inflammatory lesions and adenomas. The immunohistochemical expression in tumour tissues was correlated with the degree of infiltration through the intestinal wall. Finally, a statistical correlation was found between enzyme activity and expression obtained by Western blot in colorectal tumours when compared in the same patient. All these findings demonstrate an alteration of α(1,6)FT activity and expression in CRC. © 2008 Wiley‐Liss, Inc.