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Two‐dimensional differential gel electrophoresis/analysis of diethylnitrosamine induced rat hepatocellular carcinoma
Author(s) -
Qi Yanting,
Chen Xiaona,
Chan Chuyan,
Li Dan,
Yuan Chonggang,
Yu Fei,
Lin Marie C.,
Yew David T.,
Kung HsiangFu,
Lai Lihui
Publication year - 2008
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.23464
Subject(s) - carcinogenesis , downregulation and upregulation , hepatocellular carcinoma , blot , carcinogen , gstp1 , gel electrophoresis , proteomics , microbiology and biotechnology , chemistry , biology , glutathione s transferase , glutathione , biochemistry , cancer research , enzyme , gene
Diethylnitrosamine (DEN) is a known carcinogen that can alkylate DNA molecules. In rats, DEN‐induced hepatocellular carcinoma (HCC) model is well established. In this study, we used a two‐dimensional differential gel electrophoresis (2D‐DIGE) system and liquid chromatography/mass spectrometry/mass spectrometry to identify the differential expression protein profiles between the DEN‐induced HCC and healthy liver cells. Western blotting and semiquantitative RT‐PCR were used to further confirm the results. Seventeen differentially expressed spots were identified in DEN‐induced HCC cells. Among all, the most prominent upregulated proteins include the members of the glutathione S ‐transferase super family, aldo‐keto reductase superfamily and proteins involved in the response to oxidative stress. Downregulation was observed in 2 proteins that were known to contribute to hepatic dysfunction. This study provides the first comprehensive protein profiling of the DEN‐induced HCC in rats. This model simulates the differential protein expression of human HCC and may be useful for further understanding the mechanism of HCC tumorigenesis. © 2008 Wiley‐Liss, Inc.