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Induction of protective and therapeutic antitumor immunity by a DNA vaccine with a glioma antigen, SOX6
Author(s) -
Ueda Ryo,
Kinoshita Eiko,
Ito Rena,
Kawase Takeshi,
Kawakami Yutaka,
Toda Masahiro
Publication year - 2008
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.23366
Subject(s) - glioma , ctl* , dna vaccination , cytotoxic t cell , immunology , cd8 , immunotherapy , biology , epitope , antigen , cancer research , immune system , in vitro , immunization , genetics
We previously reported identifying SOX6 as a glioma antigen by serological screening using a testis cDNA library. Its preferential expression and frequent IgG responses in glioma patients indicate that SOX6 may be a useful target for immunotherapy. To examine whether cytotoxic T‐lymphocyte (CTL) responses specific for SOX6 to destroy glioma can be generated in vivo , we treated glioma‐bearing mice by vaccination with a plasmid DNA encoding murine full‐length SOX6 protein. Following SOX6‐DNA vaccination, CTLs specific for SOX6‐expressing glioma cells were induced, while normal autologous‐cells that had restrictedly expressed SOX6 during embryogenesis were not destroyed. Furthermore, DNA vaccination with SOX6 exerted protective and therapeutic antitumor responses in the glioma‐bearing mice. This antitumor activity was abrogated by the depletion of CD4 positive T cells and/or CD8 positive T cells. These results suggest that the SOX6 protein has multiple CTL and helper epitopes to induce antitumor activity and the effectiveness of SOX6‐DNA vaccine for the prevention and treatment of glioma. © 2008 Wiley‐Liss, Inc.