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Decreased expression of human D ‐glucuronyl C5‐epimerase in breast cancer
Author(s) -
Grigorieva Elvira,
Eshchenko Tatiana,
Rykova Valentina I.,
Chernakov Alexei,
Zabarovsky Eugene,
Sidorov Sergei V.
Publication year - 2007
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.23203
Subject(s) - breast cancer , downregulation and upregulation , western blot , malignant transformation , malignancy , proteoglycan , human breast , cancer , chemistry , messenger rna , enzyme , cancer research , blot , medicine , pathology , microbiology and biotechnology , endocrinology , biology , biochemistry , gene , extracellular matrix
D ‐glucuronyl C5‐epimerase (GLCE) is one of the key enzymes in proteoglycan biosynthesis. However, nothing is known about expression and activity of the protein in cancer. In this study, we investigated GLCE expression in human breast cancer using multipex RT‐PCR, QRT‐PCR and Western‐blot assays. In total, 21 patients without malignancy and 74 patients with breast tumor were investigated. The obtained data showed that in 82–84% of human breast tumors there is either downregulation or loss of D ‐glucuronyl C5‐epimerase mRNA expression and significant decrease of the protein content. In most cases (77%), GLCE expression was decreased also in the normal‐appearing tissue surrounding the tumor node but the protein amount was comparable to normal breast tissue. These findings represent the first data about involvement of human D ‐glucuronyl C5‐epimerase in malignant transformation. © 2007 Wiley‐Liss, Inc.