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Human PARM‐1 is a novel mucin‐like, androgen‐regulated gene exhibiting proliferative effects in prostate cancer cells
Author(s) -
Fladeby Cathrine,
Gupta Shailly N.,
Barois Nicolas,
Lorenzo Petri I.,
Simpson Jeremy C.,
Saatcioglu Fahri,
Bakke Oddmund
Publication year - 2007
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.23185
Subject(s) - lncap , prostate cancer , biology , prostate , cancer research , androgen , ectopic expression , cancer , endocrinology , medicine , microbiology and biotechnology , cell culture , hormone , genetics
In this paper we characterize hPARM‐1, the human ortholog of rat PARM‐1 (prostatic androgen‐repressed message‐1) and demonstrate its role in prostate cancer. Immunofluorescence microscopy and ultrastructural analysis revealed the localization of hPARM‐1 to Golgi, plasma membrane and the early endocytic pathway but not in lysosomes. Biochemical and deglycosylation studies showed hPARM‐1 as a highly glycosylated, mucin‐like type I transmembrane protein. Analysis of expression of hPARM‐1 in various human tissues revealed its presence in most human tissues with especially high expression in heart, kidney and placenta. Androgen controls the expression of the gene as a marked 7‐fold increase is seen in the androgen‐dependent prostate cancer cell line, LNCaP on androgen stimulation. This is further supported by its decrease in expression in CWR22 xenograft upon castration. Moreover, ectopic expression of hPARM‐1 in PC3 prostate cancer cells increased colony formation, suggesting a probable role in cell proliferation. These results suggest that hPARM‐1 may have a role in normal biology of the prostate cell and in prostate cancer. © 2007 Wiley‐Liss, Inc.