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Increased HIF1α in SDH and FH deficient tumors does not cause microsatellite instability
Author(s) -
Lehtonen Heli J.,
Mäkinen Markus J.,
Kiuru Maija,
Laiho Päivi,
Herva Riitta,
Minderhout Ivonne van,
Hogendoorn Pancras C.W.,
Cornelisse Cees,
Devilee Peter,
Laun Virpi,
Aaltonen Lauri A.
Publication year - 2007
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.22819
Subject(s) - sdhb , microsatellite instability , fumarase , cancer research , biology , germline mutation , msh2 , dna mismatch repair , citric acid cycle , mutation , cancer , endocrinology , genetics , biochemistry , enzyme , gene , metabolism , colorectal cancer , allele , microsatellite
Abstract Germline mutations in nuclear genes encoding mitochondrial enzymes fumarate hydratase (FH) and succinate dehydrogenase (subunits SDHB/C/D) have been implicated in the development of tumor syndromes referred to as hereditary leiomyomatosis and renal cell cancer (HLRCC) and hereditary paragangliomatosis (HPGL), respectively. FH and SDH are operating in the tricarboxylic acid cycle (the TCA cycle, the Krebs cycle). In the FH and SDH deficient tumors, accumulation of the substrates, fumarate and succinate, has been shown to cause stabilization of hypoxia inducible factor 1α (HIF1α). According to recent studies, HIF1α could contribute to the hypoxia induced genomic instability seen in many cancers, through repression of mismatch repair (MMR) protein MSH2. In this study, in agreement with previous works, we found HIF1α to be moderately or highly stabilized in 67% (16/24) and 77% (48/62) of HLRCC tumors and SDHB/C/D paragangliomas (PGL) and pheochromocytomas (PHEO), respectively. In addition, a set of 54 other familial and nonfamilial PGLs/PHEOs were studied. Moderately or highly stabilized HIF1α was present in 68% (26/38) of the PGLs but in PHEOs ( n = 16) no such pattern was observed. We then analyzed the suggested link between HIF1α stabilization and MSH2 repression, in HLRCC and HPGL tumor material. No microsatellite instability (MSI) or lack of MSH2 expression was, however, observed. Thus we failed to provide in vivo evidence for the proposed link between HIF1α stabilization and functional MMR deficiency, in TCAC deficient tumors. © 2007 Wiley‐Liss, Inc.