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Gene expression profiling in melanoma identifies novel downstream effectors of p14ARF
Author(s) -
Packer Leisl M.,
Pavey Sandra J.,
Boyle Glen M.,
Stark Mitchell S.,
Ayub Ana L.,
Rizos Helen,
Hayward Nicholas K.
Publication year - 2007
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.22725
Subject(s) - p14arf , biology , gene expression profiling , gene , microarray analysis techniques , cell culture , gene expression , signal transduction , cancer research , microbiology and biotechnology , genetics , tumor suppressor gene , carcinogenesis
p14ARF is inactivated by deletions/mutations in many cancer types and can suppress cell growth by both p53‐dependent and p53‐independent mechanisms. To identify novel downstream effectors of p14ARF, we used gene expression profiling as a primary screening tool to select candidates for follow up validation studies using in vitro cell‐based assays. Gene expression profiles of a panel of 35 melanoma cell lines with either wild‐type ( n = 12) or mutant ( n = 23) p14ARF were compared to identify genes associated with inactivation of p14ARF . Analysis of the microarray data identified 1,316 probe sets that were significantly ( p < 0.01) differentially expressed between the p14ARF wild‐type and mutant cell lines. Pathway analysis of these genes showed an overrepresentation of many receptor‐mediated signal transduction pathways, e.g. TGFβ, EGF, HGF, PDGF, MAPK, Wnt and integrin pathways. A number of components of these pathways, including FLRT3, RUNX2, MIG‐6 and SMURF2 were confirmed as downstream targets of p14ARF using p14ARF‐inducible cell lines and RNAi. We propose that regulation of these genes may contribute to melanoma development when p14ARF function is lost. © 2007 Wiley‐Liss, Inc.