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Functional genomics of calcium channels in human melanoma cells
Author(s) -
Deli Tamás,
Varga Norbert,
Ádám Attila,
Kenessey István,
Rásó Erzsébet,
Puskás László G.,
Tóvári József,
Fodor János,
Fehér Mónika,
Szigeti Gyula P.,
Csernoch László,
Tímár József
Publication year - 2007
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.22621
Subject(s) - melanoma , biology , wnt signaling pathway , microbiology and biotechnology , ryanodine receptor , voltage dependent calcium channel , cancer research , receptor , calcium channel , functional genomics , calcium signaling , signal transduction , computational biology , genomics , gene , genetics , genome , calcium , medicine
Abstract Ca 2+ ‐signaling of human melanoma is in the focus of intensive research since the identification of the role of WNT‐signaling in melanomagenesis. Genomic and functional studies pointed to the important role of various Ca 2+ channels in melanoma, but these data were contradictory. In the present study we clearly demonstrate, in a number of different ways including microarray analysis, DNA sequencing and immunocytochemistry, that various human melanoma cell lines and melanoma tissues overexpress ryanodine receptor type 2 (RyR2) and express P2X 7 channel proteins as compared to melanocytes. These channels, although retain some of their usual characteristics and pharmacological properties, display unique features in melanoma cells, including a functional interaction between the two molecules. Unlike P2X 7 , RyR2 does not function as a calcium channel. On the other hand, the P2X 7 receptor has an antiapoptotic function in melanoma cells, since ATP‐activation suppresses induced apoptosis, while knock down of the gene expression significantly enhances that. © 2007 Wiley‐Liss, Inc.