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Cetuximab induce antibody‐dependent cellular cytotoxicity against EGFR‐expressing esophageal squamous cell carcinoma
Author(s) -
Kawaguchi Yoshihiko,
Kono Koji,
Mimura Kousaku,
Sugai Hidemitsu,
Akaike Hidenori,
Fujii Hideki
Publication year - 2006
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.22370
Subject(s) - cetuximab , cytotoxicity , antibody dependent cell mediated cytotoxicity , cancer research , antibody , esophageal squamous cell carcinoma , basal cell , esophageal cancer , carcinoma , epidermal growth factor receptor , medicine , chemistry , cancer , immunology , monoclonal antibody , in vitro , biochemistry
To evaluate the possibility of treatment with antiepidermal growth factor receptor (EGFR) mAb, Cetuximab against esophageal squamous cell carcinoma (SCC), we performed detail analysis of the antibody‐dependent cellular cytotoxicity (ADCC) mediated by Cetuximab against esophageal SCC. Esophageal SCC cell lines with various levels of EGFR ( n = 8) were evaluated for their Cetuximab‐mediated ADCC by 51 Cr‐release assay. As a result, Cetuximab was able to induce ADCC against EGFR‐expressing esophageal SCC and the activities reflected the degree of EGFR expression on the esophageal SCC. The activities of Cetuximab‐mediated ADCC by patients' PBMC were impaired in comparison with those by healthy donors' PBMC. Moreover, the inhibition of transforming growth factor (TGF)‐β could enhance Cetuximab‐mediated ADCC against TGF‐β‐producing SCC. In conclusion, Cetuximab was able to induce ADCC against EGFR‐expressing esophageal SCC. Some modalities aiming at enhancing the Cetuximab‐mediated ADCC may be necessary for successful Cetuximab treatment of patients with esophageal SCC. © 2006 Wiley‐Liss, Inc.