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Diet supplemented with citrus unshiu segment membrane suppresses chemically induced colonic preneoplastic lesions and fatty liver in male db/db mice
Author(s) -
Suzuki Rikako,
Kohno Hiroyuki,
Yasui Yumiko,
Hata Kazuya,
Sugie Shigeyuki,
Miyamoto Shingo,
Sugawara Kuniaki,
Sumida Takashi,
Hirose Yoshinobu,
Tanaka Takuji
Publication year - 2006
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.22240
Subject(s) - aberrant crypt foci , azoxymethane , endocrinology , medicine , ratón , carcinogenesis , subcutaneous injection , biology , crypt , chemistry , colorectal cancer , cancer , colonic disease
The modulatory effects of dietary citrus unshiu segment membrane (CUSM) on the occurrence of aberrant crypt foci (ACF) and β‐catenin accumulated crypts (BCACs) were determined in male C57BL/KsJ‐ db / db ( db/db ) mice initiated with azoxymethane (AOM). Male db / db , db /+ and +/+ mice were given 5 weekly subcutaneous injections of AOM (15 mg/kg body weight), and then they were fed the diet containing 0.02%, 0.1% or 0.5% CUSM for 7 weeks. At Week 12, a significant increase in the numbers of ACF and BCAC was noted in the db / db mice in comparison with the db /+ and +/+ mice. Feeding with CUSM caused reduction in the frequency of ACF in all genotypes of mice and the potency was high in order of the db / db mice, db /+ mice and +/+ mice. The number of BCACs was also reduced by feeding with CUSM, thus resulting in a 28–61% reduction in the db/db mice, possibly due to suppression of cell proliferation activity in the lesions by feeding with CUSM‐containing diet. Clinical chemistry revealed a low serum level of triglyceride in mice fed CUSM. In addition, CUSM feeding inhibited fatty metamorphosis and fibrosis in the liver of db/db mice. Our findings show that CUSM in the diet has a chemopreventive ability against the early phase of AOM‐induced colon carcinogenesis in the db / db as well as db /+ and +/+ mice, indicating potential use of CUSM in cancer chemoprevention in obese people. © 2006 Wiley‐Liss, Inc.