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Cytokine‐induced killer cells against autologous CLL: Direct cytotoxic effects and induction of immune accessory molecules by interferon‐γ
Author(s) -
Kornacker Martin,
Moldenhauer Gerhard,
Herbst Markus,
Weilguni Edeltraud,
TitaNwa Freddy,
Harter Christoph,
Hensel Manfred,
Ho Anthony D.
Publication year - 2006
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.21994
Subject(s) - cytotoxic t cell , cytokine , immune system , biology , cd19 , immunology , peripheral blood mononuclear cell , apoptosis , interleukin 21 , cancer research , t cell , in vitro , biochemistry
Cytokine‐induced killer cells (CIK cells), coexpressing CD3 and CD56, can be expanded from peripheral blood mononuclear cells by the timed addition of interferon‐γ (IFN‐γ), IL‐2 and OKT3. The effects of CIK cells on primary, autologous CLL cells are described. We used MACS to separate CD3 + cells for expansion of CIK cell effectors and CD19 + targets from peripheral blood of 16 CLL patients. Apoptosis was assessed by measuring annexinV staining in CLL cells. After incubation of autologous CIK with CLL, specific apoptosis in CLL cells was 15%. Coincubation with irradiated CIK cells for 48 hr before adding vital CIK cells resulted in an increased ICAM‐1 expression on CLL cells and an increase in apoptosis of CLL targets (30%). These effects were mediated by IFN‐γ secretion of CIK cells. In addition to their direct cytotoxic effect, CIK cells secrete IFN‐γ that modulates the expression of adhesion molecules on CLL cells, and this enhances apoptosis induction by cytotoxic effector cells. © 2006 Wiley‐Liss, Inc.