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Chromosome 9 deletions are more frequent than FGFR3 mutations in flat urothelial hyperplasias of the bladder
Author(s) -
van Oers Johanna M.M.,
Adam Christoph,
Denzinger Stefan,
Stoehr Robert,
Bertz Simone,
Zaak Dirk,
Stief Christian,
Hofstaedter Ferdinand,
Zwarthoff Ellen C.,
van der Kwast Theodorus H.,
Knuechel Ruth,
Hartmann Arndt
Publication year - 2006
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.21958
Subject(s) - biology , bladder cancer , chromosome , cystoscopy , chromosome 9 , microbiology and biotechnology , pathology , genetics , cancer research , cancer , gene , medicine , anatomy , urinary system
Flat urothelial hyperplasias (FUHs) in patients with papillary bladder tumours frequently show deletions of chromosome 9, suggesting that FUH could be the first neoplastic step in the development of papillary bladder cancer. FGFR3 mutations are frequent in non‐invasive papillary tumours with low risk of progression. Our aim was to investigate the frequency of FGFR3 mutations and deletions of chromosomes 9p/q and 8p/q in FUH. Thirty FUH and 9 simultaneous or consecutive tumours were detected by 5‐ALA‐based photodynamic cystoscopy. DNA was isolated from frozen sections and whole genome amplification was done by I‐PEP‐PCR, followed by LOH analysis on chromosomes 8p/q and 9p/q. FGFR3 mutations were detected by SNaPshot analysis. LOH analysis on FUH revealed deletions at 9p/q (11/30, 37%) and 8p/q (3/30, 10%). FGFR3 mutations were found in 7/30 FUH (23%). Only 2 FUH showed an FGFR3 mutation without deletions of chromosome 9. In contrast, 6 FUH revealed chromosome 9 deletions but wild type FGFR3 ( p = 0.03). These results suggest that chromosome 9 deletions are the earliest genetic alterations in bladder cancer. The detection of FGFR3 mutations in FUH further supports the role of this lesion as precursor of papillary bladder cancer. © 2006 Wiley‐Liss, Inc.