Premium
Increasing cyclooxygenase‐2 ( cox‐2 ) gene expression in the progression of Barrett's esophagus to adenocarcinoma correlates with that of Bcl‐2
Author(s) -
Shimizu Daisuke,
Vallböhmer Daniel,
Kuramochi Hidekazu,
Uchida Kazumi,
Schneider Sylke,
Chandrasoma Parakrama T.,
Shimada Hiroshi,
DeMeester Tom R.,
Danenberg Kathleen D.,
Peters Jeffrey H.,
DeMeester Steven R,
Danenberg Peter V.
Publication year - 2006
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.21922
Subject(s) - adenocarcinoma , barrett's esophagus , laser capture microdissection , intraepithelial neoplasia , microdissection , carcinogenesis , medicine , esophagus , pathology , gastroenterology , intestinal metaplasia , cancer , esophageal disease , reflux esophagitis , dysplasia , cancer research , gene expression , biology , gene , reflux , disease , prostate , biochemistry
Previous studies from our laboratory and others have suggested that increased expression of cox‐2 is important in the genesis of esophageal adenocarcinoma. In vitro studies suggest that cox‐2 regulates expression of the anti‐apoptotic protein bcl‐2, thus possibly accounting for reduced apoptosis in carcinogenesis. The aim of this study was to investigate the relationship of these 2 genes in the development of Barrett's‐associated adenocarcinoma. Histologic sections from endoscopic biopsies or esophagectomy specimens were classified as non‐dysplastic Barrett's ( n = 30), intraepithelial neoplasia ( n = 12) and adenocarcinoma ( n = 48). The desired tissue was isolated by laser capture microdissection and expression levels of cox‐2 and bcl‐2 were measured by quantitative real‐time PCR (Taqman®). Gene expression levels were compared to samples of the distal esophageal squamous epithelium ( n = 55) and reflux‐esophagitis ( n = 25), without Barrett's or cancer. Expression of both bcl‐2 and cox‐2 were increased in non‐dysplastic Barrett's ( p = 0.0077, p = 0.0037), intraepithelial neoplasia ( p = 0.0053, p = 0.0220) and adenocarcinoma ( p < 0.0001, p < 0.0001) compared to squamous epithelium or reflux‐esophagitis. Furthermore, there is a significant correlation between these two genes, especially in carcinoma ( p < 0.0001). © 2006 Wiley‐Liss, Inc.