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Comparison of DNA hypermethylation patterns in different types of uterine cancer: Cervical squamous cell carcinoma, cervical adenocarcinoma and endometrial adenocarcinoma
Author(s) -
Kang Sokbom,
Kim Jae Weon,
Kang Gyeong Hoon,
Lee Sun,
Park Noh Hyun,
Song Yong Sang,
Park Sang Yoon,
Kang Soon Beom,
Lee Hyo Pyo
Publication year - 2005
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.21609
Subject(s) - adenocarcinoma , carcinoma , endometrial cancer , medicine , cervical cancer , oncology , basal cell , pathology , cancer
Abstract The incidence of cervical adenocarcinoma (CA) is rising, whereas the incidence of cervical squamous cell carcinoma (CSCC) continues to decrease. However, it is still unclear whether different molecular characteristics underlie these 2 types of cervical carcinoma. To better understand the epigenetic characteristics of cervical carcinoma, we investigated the DNA promoter hypermethylation profiles in CA and CSCC. In addition, we investigated whether DNA hypermethylation patterns might be used for the molecular diagnosis of CA and endometrial adenocarcinoma (EA). Using the bisulfite‐modification technique and methylation‐specific PCR, we examined the aberrant promoter hypermethylation patterns of 9 tumor suppressor genes ( APC , DAPK , CDH1 , HLTF , hMLH1 , p16 , RASSF1A , THBS1 and TIMP3 ) in 62 CSCCs, 30 CAs and 21 EAs. After Bonferroni correction adjustment (statistically significant at p < 0.0055), we found that the aberrant hypermethylations of CDH1 and DAPK were more frequent in CSCCs than in CAs (80.6% vs . 43.3%, p = 0.001; 77.4% vs . 46.7%, p = 0.005), whereas HLTF and TIMP3 were more frequently methylated in CAs (3.2% vs . 43.3%, p < 0.001; 8.1% vs . 53.3%, p = 0.001). The hypermethylations of RASSF1A and APC were more frequent in CAs than in CSCCs, but this was not significant (9.7% vs . 33.3%, p = 0.008; and 14.5% vs . 40.0%, respectively, p = 0.009). In addition, RASSF1A hypermethylation was significantly more frequent in EAs than in CAs (81.0% vs . 33.3%, p = 0.001). In conclusion, the existence of these unique methylation patterns in these cancers suggests that their tumorigenesis may involve different epigenetic mechanisms. © 2005 Wiley‐Liss, Inc.