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A point mutation in the NFYC gene generates an antigenic peptide recognized by autologous cytolytic T lymphocytes on a human squamous cell lung carcinoma
Author(s) -
Takenoyama Mitsuhiro,
Baurain JeanFrançis,
Yasuda Manabu,
So Tomoko,
Sugaya Masakazu,
Hanagiri Takeshi,
Sugio Kenji,
Yasumoto Kosei,
Boon Thierry,
Coulie Pierre G.
Publication year - 2005
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.21594
Subject(s) - ctl* , antigen , biology , cancer research , tumor antigen , cd8 , immunology , immunotherapy , immune system
We have identified an antigen recognized by cytolytic T lymphocytes (CTL) on the autologous tumor cells of a nonsmall cell lung cancer patient. The antigenic peptide, presented by HLA‐B*5201 molecules, was encoded by a mutated sequence in the gene coding for the C subunit of transcription factor NF‐Y. The mutation was present in the tumor sample from which the cell line was derived, and appeared to be unique to the tumor of this patient. In a lymph node draining the tumor, precursors of CTL recognizing the autologous tumor cells were detected at a frequency of about 1/30,000 of the CD8 cells, and 85% of them recognized the mutated NF‐YC peptide, suggesting that the patient mounted a T cell response against this antigen. These results strengthened the notion that unique tumor‐specific antigens are highly represented not only in melanoma but also in other types of tumors, like nonsmall cell lung cancer. © 2005 Wiley‐Liss, Inc.