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Gene expression profiles relate to SS18/SSX fusion type in synovial sarcoma
Author(s) -
Fernebro Josefin,
Francis Princy,
Edén Patrik,
Borg Åke,
Panagopoulos Ioannis,
Mertens Fredrik,
VallonChristersson Johan,
Åkerman Måns,
Rydholm Anders,
Bauer Henrik CF,
Mandahl Nils,
Nilbert Mef
Publication year - 2005
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.21475
Subject(s) - synovial sarcoma , fusion gene , biology , gene expression , gene , microarray analysis techniques , microarray , gene expression profiling , pathology , microbiology and biotechnology , cancer research , sarcoma , genetics , medicine
We applied 27k spotted cDNA microarray slides to assess gene expression profiles in 26 samples from 24 patients with synovial sarcomas (SS). The data were analyzed in relation to histopathologic type, cytogenetic aberrations, gene fusion type and development of distant metastases. Supervised analysis based on gene fusion type in 12 SS with SS18/SSX1 and 9 with SS18/SSX2 revealed significant differences in gene expression profiles. Among the discriminators were several genes that have previously been found to be upregulated in SS, including AXL , ZIC2, SPAG7, AGRN, FOXC1, NCAM1 and multiple metallothioneins. Histopathology and degree of cytogenetic complexity did not significantly influence expression, whereas a genetic signature that related to development of metastases could be discerned, albeit with a high false‐positive rate. In conclusion, our findings demonstrate differentially expressed genes for the 2 major gene fusion variants in SS, SS18/SSX1 and SS18/SSX2 , and thereby suggest that these result in different downstream effects. © 2005 Wiley‐Liss, Inc.