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Protein expression profiling identifies maspin and stathmin as potential biomarkers of adenoid cystic carcinoma of the salivary glands
Author(s) -
Nakashima Dai,
Uzawa Katsuhiro,
Kasamatsu Atsushi,
Koike Hirofumi,
Endo Yosuke,
Saito Kengo,
Hashitani Susumu,
Numata Tsutomu,
Urade Masahiro,
Tanzawa Hideki
Publication year - 2005
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.21318
Subject(s) - maspin , stathmin , adenoid cystic carcinoma , immunohistochemistry , pathology , salivary gland , biology , cancer research , proteomics , carcinoma , medicine , cancer , metastasis , gene , biochemistry , genetics
Adenoid cystic carcinoma (ACC) is one of the most common malignant tumors of the salivary glands. It tends to grow slowly but is associated with a poor prognosis compared to other malignant salivary gland tumors. To identify specific markers of ACC, we examined protein expression profiling in ACC xenograft and normal salivary glands (NSG) using fluorescent 2‐dimensional differential in‐gel electrophoresis (2‐D‐DIGE), an emerging technique for comparative proteomics, that improves the reproducibility and reliability of differential protein expression analysis between the samples. To identify the proteins, matrix‐assisted laser desorption/ionization time‐of‐flight peptide mass fingerprinting was carried out. Using these strategies, we detected 4 upregulated proteins and 5 downregulated proteins in ACC xenograft. Maspin and stathmin were selected for further analyses. Western blotting and immunohistochemical staining showed a higher expression of these proteins in ACC xenograft and clinical ACC tissue compared to NSG. Furthermore, Expression of these proteins was correlated with the histologic grading of ACC ( n = 10). Therefore, our data indicate that maspin and stathmin may be not only useful biomarkers of ACC but also markers of biologic behavior in this tumor. © 2005 Wiley‐Liss, Inc.