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Biases in human papillomavirus genotype prevalence assessment associatedwith commonly used consensus primers
Author(s) -
Chan Paul K. S.,
Cheung TakHong,
Tam Ann O. Y.,
Lo Keith W. K.,
Yim SoFan,
Yu May M. Y.,
To KaFai,
Wong YickFu,
Cheung Jo L. K.,
Chan Denise P. C.,
Hui Mamie,
Ip Margaret
Publication year - 2005
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.21299
Subject(s) - genotype , cervical cancer , human papillomavirus , primer (cosmetics) , virology , medicine , basal cell , cancer , biology , oncology , genetics , gene , chemistry , organic chemistry
Consensus primers targeting human papillomaviruses (HPVs) have biases in sensitivity toward certain HPV types. We applied 3 primer sets (GP5+/6+, MY09/11, PGMY09/11) in parallel on 120 Chinese cervical cancer specimens. GP5+/6+ exhibited a poor sensitivity for HPV52, for which the prevalence among squamous cell cervical cancer was underestimated from 14.6% to 0%. The fact that HPV52 should rank second in prevalence among squamous cell cervical carcinoma in Hong Kong could be missed if GP5+/6+, a worldwide commonly used primer set, was selected for HPV detection. Biases in HPV type‐specific sensitivity may result in misprioritization of vaccine candidates. © 2005 Wiley‐Liss, Inc.