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Antiinvasive effect of xanthohumol, a prenylated chalcone present in hops ( Humulus lupulus L.) and beer
Author(s) -
Vanhoecke Barbara,
Derycke Lara,
Van Marck Veerle,
Depypere Herman,
De Keukeleire Denis,
Bracke Marc
Publication year - 2005
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.21249
Subject(s) - xanthohumol , humulus lupulus , in vitro , in vivo , biochemistry , chemistry , hop (telecommunications) , biology , pharmacology , food science , computer network , microbiology and biotechnology , pepper , computer science
The female inflorescences of the hop plant ( Humulus lupulus L.) are essential during brewing to add taste and flavor to beer and to stabilize beer foam. Xanthohumol, the main prenylated chalcone in hops, was investigated for its antiinvasive activity on human breast cancer cell lines (MCF‐7 and T47‐D) in vitro . Xanthohumol was able to inhibit the invasion of MCF‐7/6 cells at 5 μM in the chick heart invasion assay and of T47‐D cells in the collagen invasion assay. Xanthohumol inhibited growth of MCF‐7/6 and T47‐D cells, but not of chick heart cells. Moreover, it induced apoptosis of these tumor cells as demonstrated by the cleavage of nuclear PARP after 48 hr treatment. To probe the mechanism of the antiinvasive effect of xanthohumol, involvement of the E‐cadherin/catenin invasion‐suppressor complex was investigated. An aggregation assay demonstrated stimulation of aggregation of MCF‐7/6 cells in the presence of 5 μM xanthohumol and this could be completely inhibited by an antibody against E‐cadherin. Xanthohumol upregulates the function of the E‐cadherin/catenin complex and inhibits invasion in vitro , indicating a possible role as an antiinvasive agent in vivo as well. © 2005 Wiley‐Liss, Inc.