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A novel mouse model for segmental orthotopic colon cancer
Author(s) -
Alencar Herlen,
King Ray,
Funovics Martin,
Stout Charles,
Weissleder Ralph,
Mahmood Umar
Publication year - 2005
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.21185
Subject(s) - colorectal cancer , genetically modified mouse , pathology , magnetic resonance imaging , lesion , cancer , medicine , transgene , biology , cancer research , radiology , gene , biochemistry
Spontaneous colon tumor mouse strains offer numerous advantages in modeling disease. However, the wide temporal window in which lesions form and the stochastic nature of lesion location require larger cohorts for assessment of disease modulation. Reliable, reproducible and inexpensive mouse models of early‐stage and invasive cancer would add to existing transgenic models. We show a new method for the creation of orthotopic murine tumors centered in the mucosal and submucosal layers anywhere in the colon, allowing creation of lesions of known age, location and extent. The system overcomes the disadvantages of heterotopic implantation and allows evaluation of lesions distally in the colon as well as proximally, thereby providing an additional method to study the effects of regionality. Invasion, host vascularization and application to disparate cell lines are demonstrated. Noninvasive imaging with magnetic resonance and colonoscopy, allowed in part by the tumor location, show potential applications of this approach. © 2005 Wiley‐Liss, Inc.