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Loss of heterozygosity of 1p in uveal melanomas with monosomy 3
Author(s) -
Häusler Thomas,
Stang Andreas,
Anastassiou Gerasimos,
Jöckel KarlHeinz,
Mrzyk Stefanie,
Horsthemke Bernhard,
Lohmann Dietmar R.,
Zeschnigk Michael
Publication year - 2005
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.21086
Subject(s) - monosomy , loss of heterozygosity , biology , melanoma , chromosome 7 (human) , chromosome 3 , aneuploidy , chromosome , cancer research , pathology , cytogenetics , genetics , karyotype , allele , medicine , gene
Gains and losses of chromosomes 1, 3, 6 and 8 are nonrandom chromosomal aberrations in uveal melanoma. Monosomy 3 is the most frequent abnormality and is associated with poor prognosis. To identify regions of allelic loss on the short arm of chromosome 1 and to investigate if these alterations contribute to uveal melanoma progression, we performed microsatellite analysis of 10 loci in 70 uveal melanomas. A total of 51 tumors were obtained from patients with clinical follow‐up data, 19 tumors were from recent patients without follow‐up. Loss of heterozygosity (LOH) of at least 1 marker was more frequent in tumors with monosomy 3 (40%) than in tumors with disomy 3 (10%). In particular, loss of the entire short arm of chromosome 1 was only observed in tumors with monosomy 3 ( p = 0.0001). By comparing the extent of 1p LOH in all tumors with monosomy 3, we were able to define a smallest region of overlap (SRO) of approximately 55 Mb, which is flanked by markers D1S507 and D1S198 . On the basis of our data and published cytogenetic data, we propose that 1p31 harbors genes involved in the progression of uveal melanoma with monosomy 3. © 2005 Wiley‐Liss, Inc.