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Regulation of CTL responses to MHC‐restricted class I peptide of the gp70 tumour antigen by splenic parenchymal CD4 + T cells in mice failing immunotherapy with DISC–mGM‐CSF
Author(s) -
Ahmad Murrium,
Rees Robert C.,
McArdle Stephanie E.,
Li Geng,
Mian Shahid,
Entwisle Claire,
Loudon Peter,
Ali Selman A.
Publication year - 2005
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.20976
Subject(s) - ctl* , immunotherapy , biology , cytotoxic t cell , immunology , cytokine , parenchyma , population , spleen , antigen , t lymphocyte , immune system , cancer research , cd8 , in vitro , medicine , biochemistry , botany , environmental health
Direct intratumour injection of the disabled infectious single‐cycle–herpes simplex virus–encoding murine granulocyte/macrophage colony‐stimulating factor (DISC‐HSV–mGM‐CSF) into established colon carcinoma CT26 tumours induced complete tumour rejection in up to 70% of treated animals (regressors), while the remaining mice developed progressive tumours (progressors). This murine Balb/c model was used to dissect the cellular mechanisms involved in tumour regression or progression following immunotherapy. CTLs were generated by coculturing lymphocytes and parenchymal cells from the same spleens of individual regressor or progressor animals in the presence of the relevant AH‐1 peptide derived from the gp70 tumour‐associated antigens expressed by CT26 tumours. Tumour regression was correlated with potent CTL responses, spleen weight and cytokine (IFN‐γ) production. Conversely, progressor splenocytes exhibited weak to no CTL activity and poor IFN‐γ production, concomitant with the presence of a suppressor cell population in the progressor splenic parenchymal cell fraction. Further fractionation of this parenchymal subpopulation demonstrated that cells inhibitory to the activation of AH‐1‐specific CTLs, restimulated in vitro with peptide, were present in the nonadherent parenchymal fraction. In vitro depletion of progressor parenchymal CD3 + /CD4 + T cells restored the CTL response of the cocultured splenocytes (regressor lymphocytes and progressor parenchymal cells) and decreased the production of IL‐10, suggesting that CD3 + CD4 + T lymphocytes present in the parenchymal fraction regulated the CTL response to AH‐1. We examined the cellular responses associated with tumour rejection and progression, identifying regulatory pathways associated with failure to respond to immunotherapy. © 2005 Wiley‐Liss, Inc.