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CpG island methylation in aberrant crypt foci and cancers from the same patients
Author(s) -
Luo Liping,
Chen Weidong,
Pretlow Theresa P.
Publication year - 2005
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.20936
Subject(s) - methylation , dna methylation , cpg site , aberrant crypt foci , carcinogenesis , colorectal cancer , cancer research , biology , cancer , pathology , epigenetics , crypt , bisulfite sequencing , medicine , gene , genetics , gene expression , colonic disease , endocrinology
Aberrant methylation of CpG islands is a common alteration in human colon cancer. Methylation of only a limited number of loci has been studied in aberrant crypt foci (ACF), the earliest identified premalignant lesions in the colon, and in only a limited number of lesions from the same patients. Methylation‐specific PCR was used to analyze 35 ACF, samples of normal crypts with the same number of crypts as were in each ACF and 22 cancers from the same patients. Aberrant methylation in cellular retinol‐binding protein 1 ( CRBP1), MINT31 or H‐cadherin ( CDH13 ) was identified in 19 of 35 (54%) ACF. Hypermethylation of CRBP1, MINT31 or CDH13 was more frequent (15 of 22, 68%) in cancers. DNA methylation of CRBP1, MINT31 or CDH13 was not correlated between cancers and ACF from the same patients. Aberrant methylation was not correlated with patient age, number of crypts in the ACF or cancer stage. These results suggest that hypermethylation in the promoter region of some genes is an independent event, occurs early in human colon tumorigenesis and may play an important role during the transformation and progression of some lesions to colon cancers. © 2005 Wiley‐Liss, Inc.