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Role of p53 status in chemosensitivity determination of cancer cells against histone deacetylase inhibitor sodium butyrate
Author(s) -
Joseph Jeena,
Wajapeyee Narendra,
Somasundaram Kumaravel
Publication year - 2005
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.20842
Subject(s) - sodium butyrate , apoptosis , histone deacetylase inhibitor , histone deacetylase , trichostatin a , cancer research , cell cycle checkpoint , cell cycle , cancer cell , butyrate , cell growth , biology , hdac11 , dna damage , histone , null cell , cancer , cell culture , dna , biochemistry , genetics , fermentation
Histone deacetylases inhibitors (HDIs) induce growth arrest and apoptosis in a variety of human cancer cells. Sodium butyrate (NaB), a histone deacetylase inhibitor, has been shown to cause a G 1 cell cycle arrest by inducing p21 WAF1/CIP1 in a p53 ‐independent manner. In this report, we present evidence for activation of p53 pathway by NaB and its role in the NaB‐mediated growth suppression. Addition of NaB increased the levels of p53 involving a p14 ARF ‐dependent post‐transcriptional mechanism. NaB induced p53 is functional as it activated p53 ‐specific reporter, induced the level of p21 WAF1/CIP1 , inhibited cellular DNA synthesis and induced apoptosis. By using HPV 16 E6 stable transfectants as well as p53 null cancer cells, we show that NaB suppresses the growth of WT p53 containing cells more efficiently. NaB inhibited DNA synthesis to similar extent both in the presence and absence of p53. However, NaB treatment lead to a major G 2 /M arrest of cells in the presence of p53, while cells without wild‐type p53 accumulated mainly in G 1 phase of the cell cycle. Furthermore, apoptosis induction by NaB is greatly reduced in the absence of p53. These results suggest that p53 pathway mediates in part growth suppression by NaB and the p53 status may be an important determinant of chemosensitivity in HDI based cancer chemotherapy. © 2005 Wiley‐Liss, Inc.