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c‐ myc ‐induced hepatocarcinogenesis in the absence of IGF‐I receptor
Author(s) -
Cadoret Axelle,
DesboisMouthon Christèle,
Wendum Dominique,
Leneuve Patricia,
Perret Christine,
Tronche François,
Housset Chantal,
Holzenberger Martin
Publication year - 2004
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.20805
Subject(s) - hccs , autocrine signalling , paracrine signalling , biology , cancer research , carcinogenesis , receptor , genetically modified mouse , endocrinology , medicine , hepatocellular carcinoma , transgene , cancer , gene , genetics
Numerous tumours, including hepatocarcinomas, produce IGFs, and some depend on these growth factors in a paracrine or autocrine fashion. We have shown that c‐ myc ‐induced experimental hepatocarcinogenesis is associated with enhanced production of IGF‐II. To assess the role of the IGF‐I receptor (IGF‐IR) in hepatocarcinogenesis, we generated conditional mutant mice that overexpressed c‐ myc and were knocked out for IGF‐IR specifically in the liver. We compared these mice with littermate controls that also overexpressed c‐ myc but had wild‐type IGF‐IR alleles. We found that the pretumoral phase, induced by early c‐ myc expression and characterised by increased cell proliferation, was largely unaffected by the lack of IGF‐IR. To our further surprise, hepatocellular carcinomas (HCCs) lacking IGF‐IR readily developed and progressed at the same rate as control HCCs. At 9 months, all c‐ myc transgenic mice displayed well‐differentiated multifocal tumours, regardless of whether their livers—and their tumours—were able to produce IGF‐IR. Levels of IRS‐1 and IRS‐2 were elevated in all tumours in the presence or absence of IGF‐IR, suggesting that the signalling pathway downstream of IGF‐IR is activated via IGF‐IR‐independent mechanisms in HCC. In conclusion, the deregulation of IGF signalling pathways, which often occurs during liver tumorigenesis, does not necessarily require IGF‐IRs, and hepatic IGF‐IR alone may not play a determinant role in c‐ myc ‐induced hepatocarcinogenesis. © 2004 Wiley‐Liss, Inc.