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Oxidative DNA damage and antioxidant vitamin level: Comparison among lung cancer patients, healthy smokers and nonsmokers
Author(s) -
Gackowski Daniel,
Kowalewski Janusz,
Siomek Agnieszka,
Olinski Ryszard
Publication year - 2005
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.20700
Subject(s) - lung cancer , medicine , oxidative stress , cancer , vitamin c , antioxidant , vitamin e , physiology , gastroenterology , endocrinology , vitamin , chemistry , biochemistry
In the present study, we examined whether the level of 8‐oxo‐7,8‐dihydro‐2′‐deoxyguanosine (8‐oxodGuo) in leukocyte DNA is higher in lung cancer patients compared to controls. Factors that may influence oxidative stress, such as antioxidant vitamins, were also determined. These parameters were analyzed in 4 groups of subjects: smokers with lung cancer, ex‐smokers with lung cancer, healthy smokers with comparable smoking status and healthy nonsmokers. The 8‐oxodGuo mean level in leukocytes of lung cancer patients reached values of 9.22/10 6 dGuo molecules (smokers) and 11.16/10 6 dGuo molecules (ex‐smokers). These values were significantly higher than in DNA of healthy smokers and nonsmokers, where mean levels reached 6.99/10 6 dGuo molecules and 5.98/10 6 dGuo molecules, respectively. Mean levels of vitamin C in the plasma of controls and lung cancer patients were 56.17 μM (nonsmokers), 26.34 μM (healthy smokers), 23.83 μM (cancer patients, smokers) and 29.19 μM (cancer patients, ex‐smokers). The difference between nonsmokers and the 3 other groups was statistically significant. Vitamin E level was significantly reduced in the plasma of cancer patients (smokers 19.94 μM, ex‐smokers 19.59 μM) compared to healthy smokers (28.93 μM). No changes in vitamin A concentration were found. Our results suggest that a high level of 8‐oxodGuo in leukocyte DNA and a low concentration of vitamin E in the blood may predict lung cancer risk. However, it is also possible that these phenomena may simply result from disease development. © 2004 Wiley‐Liss, Inc.