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Activation of gef‐h1 , a guanine nucleotide exchange factor for RhoA, by DNA transfection
Author(s) -
Brecht Marcus,
Steenvoorden Agatha C.M.,
Collard John G.,
Luf Susanne,
Erz Dorothee,
Bartram Claus R.,
Janssen Johannes W.G.
Publication year - 2004
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.20626
Subject(s) - guanine nucleotide exchange factor , pleckstrin homology domain , rhoa , transfection , gtpase , biology , guanine , microbiology and biotechnology , homology (biology) , dna , gene , nucleotide , genetics , signal transduction
Several oncogenes isolated by the NIH/3T3 transformation assay, i.e. , dbl , dbs , lbc , lfc , lsc , net , ost and tim , contain a Dbl homology (DH) and a pleckstrin‐homology (PH) domain and act as GEFs (guanine nucleotide exchange factors) for Rho‐like GTPases. In a search for genes with oncogenic potential in DNA from the monocytic leukaemia cell line U937, we identified an amino‐terminal truncated form of gef‐h1 , a gene encoding a GEF for RhoA. These data support the idea that a systematic search for mutations and/or deletions of GEFs in human cancer is promising.