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Prostaglandin E and prostacyclin receptor expression in tumor and host tissues from MCG 101‐bearing mice: A model with prostanoid‐related cachexia
Author(s) -
Wang Wenhua,
Andersson Marianne,
Lõnnroth Christina,
Svanberg Elisabeth,
Lundholm Kent
Publication year - 2005
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.20539
Subject(s) - prostacyclin , prostanoid , cachexia , prostaglandin , receptor , endocrinology , medicine , prostaglandin antagonist , biology , cancer
Preclinical and clinical studies in our laboratory have suggested that prostaglandin (PG) E 2 is involved in anorexia and cachexia development, although the role of COX pathways on the pathogenesis of cancer cachexia remains to be clarified. Expressions of PGE (EP 1 , EP 2 , EP 3α,β,γ and EP 4 ) and PGI (IP) receptors in the central nervous system (brain cortex, hypothalamus and brain stem), in peripheral (liver, white adipose tissue and skeletal muscle) and tumor tissue from MCG‐101‐bearing mice with and without indomethacin treatment were investigated by RT‐PCR and immunohistochemistry. Expression of EP 1 in the liver and EP 4 receptor in white adipose tissue were upregulated and responded to indomethacin treatment, while downregulated expression of EP 3 in skeletal muscle from tumor‐bearing mice was unresponsive to indomethacin treatment despite improved carcass weight. Expression of EP and IP receptors in brain and tumor tissue from tumor‐bearing mice were neither related nor responsive to systemic PGE 2 levels including increased IL‐1bβ, IL‐6 and TNF‐aα host activities. The expression IP receptor in CNS, peripheral tissue and tumor tissue was unchanged by cachexia development. Our results suggest that transcription of EP receptors in liver, fat and skeletal muscle tissue may be a control level for host metabolic alterations during tumor progression, while overall EP and IP receptor expression in CNS did not indicate an important control level for appetite regulation in MCG 101‐bearing mice despite prostanoid related anorexia. © 2005 Wiley‐Liss, Inc.