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Immunohistochemical investigation of SV40 large T antigen in Hodgkin and non‐Hodgkin's lymphoma
Author(s) -
Brousset Pierre,
de Araujo Valquiria,
Gascoyne Randy D.
Publication year - 2004
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.20397
Subject(s) - lymphoma , immunohistochemistry , antigen , pathology , tissue microarray , virus , biology , medicine , virology , immunology
DNA sequences from simian virus 40 (SV40) have been detected in various human tumors, including non‐Hodgkin's lymphomas (NHLs), by highly sensitive PCR techniques. However, there is a strong debate as to whether SV40 is present in lymphoma cells. Using immunohistochemistry and tissue microarrays, we investigated a series of French and Canadian cases of Hodgkin's lymphomas (HLs) and NHLs and tried to detect the SV40 large T antigen using routine paraffin sections with standard and highly sensitive catalyzed system amplification methods. None of the cases of HLs ( n = 250) or NHLs ( n = 232) were found to contain a single large T antigen‐positive cell, whereas 2 positive controls were repeatedly stained. Therefore, our results do not support the hypothesis that SV40 is implicated in the etiology of human lymphomas. © 2004 Wiley‐Liss, Inc.