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Extensive analysis of the 7q31 region in human prostate tumors supports TES as the best candidate tumor suppressor gene
Author(s) -
Chêne Laurent,
Giroud Christiane,
Desgrandchamps François,
BocconGibod Laurent,
Cussenot Olivier,
Berthon Philippe,
Latil Alain
Publication year - 2004
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.20337
Subject(s) - loss of heterozygosity , biology , tumor suppressor gene , prostate , suppressor , candidate gene , gene , stromal cell , cancer research , immunohistochemistry , gene expression , microbiology and biotechnology , genetics , carcinogenesis , cancer , allele , immunology
Loss of heterozygosity (LOH) on chromosome arm 7q31 is found in many prostate tumors. Such alterations are generally associated with inactivation of tumor suppressor genes. It has been shown previously that the main region of LOH at 7q31 spans the interval between the D7S486 and D7S2460 microsatellite loci, which contains several candidate tumor suppressor genes (TSG) such as TES , CAV2 , CAV1 , MET , CAPZA2 , ST7 and WNT2 . We tested 41 human sporadic prostate tumors for 7q31 LOH by using 5 polymorphic markers overlapping the critical region and used a real‐time reverse transcriptase‐polymerase chain reaction (RT‐PCR) assay to study the expression of the 7 candidate TSGs located in this genomic region. We found that CAV1 , CAV2 , MET and TES mRNA expression was lower in prostate tumors than in normal prostate tissues. Our immunohistochemical results and previously published data on the compartmental expression of these messenger RNAs in stromal and epithelial cells suggest that TES is the best candidate tumor suppressor gene at 7q31. © 2004 Wiley‐Liss, Inc.