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Bone metastasis: Osteoblasts affect growth and adhesion regulons in prostate tumor cells and provoke osteomimicry
Author(s) -
Knerr Kerstin,
Ackermann Karin,
Neidhart Tanja,
Pyerin Walter
Publication year - 2004
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.20223
Subject(s) - osteoblast , metastasis , bone metastasis , prostate cancer , cancer research , biology , crosstalk , prostate , cell adhesion , primary tumor , microbiology and biotechnology , cell , cancer , in vitro , genetics , optics , physics
Bone metastasis is the primary cause of death in human prostate cancer. Disseminated from primary tumor and distributed via the bloodstream, a proportion of prostate carcinoma cells eventually reach the skeleton and develop into metastases, requiring adhesion to inner bone surfaces lined by osteoblasts. The crosstalk of tumor cells with osteoblasts is a critical but poorly characterized step in the metastatic process. Using an in vitro metastasis model system, we have been examining effects of osteoblast‐released factors on gene expression of prostate carcinoma cells. Here, we show by large‐scale transcript profiling and quantitative RT‐PCR that osteoblast‐released factors target in particular the proliferation and adhesion regulons of tumor cells. Genes encoding components of the cell‐cycle control machinery and connected pathways are predominantly repressed and cell proliferation is slowed down, resembling in vivo observations assumed to render commonly used chemotherapeutic measures ineffective. Genes encoding anchoring junction components are predominantly elevated, and the adhesion properties of tumor cells are altered. Moreover, prostate carcinoma cells are provoked to undergo osteomimicry, i.e., to express bone cell–related genes. The data indicate that the crosstalk with osteoblasts induces expressional changes in prostate carcinoma cells favoring the bone colonization process. © 2004 Wiley‐Liss, Inc.