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Gene expression analysis in Interleukin‐12‐induced suppression of mouse mammary carcinoma
Author(s) -
Shi Xiaoyan,
Liu Jianguo,
Xiang Zhaoying,
Mitsuhashi Maki,
Wu Rita S.,
Ma Xiaojing
Publication year - 2004
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.20145
Subject(s) - metastasis , cancer research , cytotoxic t cell , malignancy , biology , immunotherapy , breast cancer , breast carcinoma , carcinoma , immunology , mammary tumor , mammary gland , melanoma , cancer , pathology , medicine , immune system , in vitro , genetics , biochemistry
Interleukin‐12 (IL‐12) has potent antitumor activities via natural killer cells and cytotoxic T lymphocytes. However, the molecular mechanisms whereby IL‐12 induces tumoricidal activities are poorly understood. Here, we report the genome‐wide analysis of gene expression in a primary murine mammary carcinoma model that resembles human breast cancer, following the therapeutic application of recombinant IL‐12, which restricted tumor growth and metastasis. IL‐12 was able to curtail neovascularization in the tumor as well as enhance the number of tumor‐infiltrating lymphocytes. Comprehensive examination of global gene expression revealed IL‐12‐induced molecular changes associated with tumor regression and reduced lung metastasis, thus providing a high‐resolution snapshot of a host response against a developing malignancy and a rich source of potential targets for therapeutic intervention of breast cancer. © 2004 Wiley‐Liss, Inc.