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Highly efficient DNA incorporation of intratumourally injected [ 125 I]iododeoxyuridine under thymidine synthesis blocking in human glioblastoma xenografts
Author(s) -
Buchegger Franz,
Adamer Florence,
Schaffland Andreas Oliver,
Kosinski Marek,
Grannavel Carine,
Dupertuis Yves Marc,
de Tribolet Nicolas,
Mach JeanPierre,
Delaloye Angelika Bischof
Publication year - 2004
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.20112
Subject(s) - idoxuridine , thymidine , biodistribution , chemistry , microbiology and biotechnology , spleen , cancer research , dna , biology , biochemistry , immunology , in vitro , virus
Intratumoural (i.t.) injection of radio‐iododeoxyuridine (IdUrd), a thymidine (dThd) analogue, is envisaged for targeted Auger electron‐ or beta‐radiation therapy of glioblastoma. Here, biodistribution of [ 125 I]IdUrd was evaluated 5 hr after i.t. injection in subcutaneous human glioblastoma xenografts LN229 after different intravenous (i.v.) pretreatments with fluorodeoxyuridine (FdUrd). FdUrd is known to block de novo dThd synthesis, thus favouring DNA incorporation of radio‐IdUrd. Results showed that pretreatment with 2 mg/kg FdUrd i.v. in 2 fractions 0.5 hr and 1 hr before injection of radio‐IdUrd resulted in a mean tumour uptake of 19.8% of injected dose (% ID), representing 65.3% ID/g for tumours of approx. 0.35 g. Tumour uptake of radio‐IdUrd in non‐pretreated mice was only 4.1% ID. Very low uptake was observed in normal nondividing and dividing tissues with a maximum concentration of 2.9% ID/g measured in spleen. Pretreatment with a higher dose of FdUrd of 10 mg/kg prolonged the increased tumour uptake of radio‐IdUrd up to 5 hr. A competition experiment was performed in FdUrd pretreated mice using i.t. co‐injection of excess dThd that resulted in very low tumour retention of [ 125 I]IdUrd. DNA isolation experiments showed that in the mean >95% of tumour 125 I activity was incorporated in DNA. In conclusion, these results show that close to 20% ID of radio‐IdUrd injected i.t. was incorporated in tumour DNA after i.v. pretreatment with clinically relevant doses of FdUrd and that this approach may be further exploited for diffusion and therapy studies with Auger electron‐ and/or beta‐radiation‐emitting radio‐IdUrd. © 2004 Wiley‐Liss, Inc.