Premium
Identifying progression‐associated genes in adult T‐cell leukemia/lymphoma by using oligonucleotide microarrays
Author(s) -
Tsukasaki Kunihiro,
Tanosaki Sakae,
DeVos Sven,
Hofmann Wolf K.,
Wachsman William,
Gombart Adrian F.,
Krebs Johannes,
Jauch Anna,
Bartram Claus R.,
Nagai Kazuhiro,
Tomonaga Masao,
Said Jonathan W.,
Koeffler H. Phillip
Publication year - 2004
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.20028
Subject(s) - biology , gene , dna microarray , microarray , acute leukemia , microbiology and biotechnology , gene expression , chronic lymphocytic leukemia , immune system , cancer research , leukemia , immunology , genetics
Adult T‐cell leukemia/lymphoma (ATL) is associated with human T‐lymphotropic virus type‐1 (HTLV‐1). To understand the changes in expression that occur in the progression of chronic phase of ATL to acute crisis, the gene expression profiles of fresh ATL cells were compared in 4 pairs of samples (progression of chronic to acute phase in 3 patients, as well as 1 typical chronic phase sample vs . 1 typical acute phase sample) using high‐density oligonucleotide DNA arrays. We identified 203 genes that were commonly upregulated in acute vs . chronic phase samples including ribosomal proteins, proteosome subunits, eukaryotic translation factors, immunophilins, heat shock proteins and genes important for DNA replication. Additionally, we identified 91 commonly downregulated genes including immune molecules related to MHC and a phosphatase. Several of the genes were previously identified to be associated with the Tax protein of HTLV‐1. Some of the upregulated genes were located in amplified regions identified by comparative genomic hybridization in the corresponding chronic/acute ATL sample. Using real‐time quantitative PCR, we confirmed the array‐results in those specimens analyzed by microarray. These results demonstrated that distinct sets of genes that are known to be critical in cellular transformation and/or activation are up‐ or down‐regulated during the transition to the acute phase of ATL. © 2004 Wiley‐Liss, Inc.