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Identification and characterization of 9D7, a novel human protein overexpressed in renal cell carcinoma
Author(s) -
Klade Christoph S.,
Dohnal Alexander,
Fürst Walter,
Sommergruber Wolfgang,
Heider KarlHeinz,
Gharwan Helen,
Ratschek Manfred,
Adolf Günther R.
Publication year - 2001
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.1582
Subject(s) - biology , complementary dna , exon , microbiology and biotechnology , cdna library , gene , renal cell carcinoma , messenger rna , in situ hybridization , open reading frame , rna splicing , southern blot , peptide sequence , genetics , pathology , rna , medicine
With the objective of discovering novel tumor‐associated antigens of the cancer/testis type, we compared the transcriptional profiles of renal cell carcinoma (RCC) and non‐tumorous kidney and further screened for genes expressed in RCC and testis, but not other normal tissues. In a first step, a representational difference analysis library consisting of approximately 1,900 RCC cDNA clones was generated. Clones were then spotted onto filters and hybridized with cDNA probes derived from a testis‐specific cDNA library, a pool of RCCs and a pool of 10 healthy normal tissues, respectively. Based on strong hybridization signals with both RCC and testis, but not normal tissue probes, 185 clones were sequenced and annotated. After EST‐database comparison, 35 clones were selected for experimental analysis, including conventional and quantitative RT‐PCR as well as Northern blotting. Clone 9D7 showed strong mRNA expression in RCC as well as in several other major tumor types. In normal tissues there was little or no mRNA expression with the exception of heart. 9D7 was cloned to full‐size and found to represent a novel human gene containing 5 exons residing on chromosome 14. Alternative splicing within exon 1 generates 2 open‐reading‐frames consisting of 717 or 435 bp corresponding to predicted proteins of 239 or 145 amino acids. 9D7 shows high homology (227/239 amino acids or 95% identity) to a growth factor‐inducible gene of Rattus norvegicus involved in apoptosis. In situ hybridization as well as immunohistochemical analysis using 9D7‐specific antisera confirmed overexpression of 9D7 in RCCs as compared to normal kidney tissue. © 2002 Wiley‐Liss, Inc.