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Chromosomal aberrations in colorectal cancers and liver metastases analyzed by comparative genomic hybridization
Author(s) -
Aragane Hideki,
Sakakura Chouhei,
Nakanishi Masayoshi,
Yasuoka Rie,
Fujita Yoshifumi,
Taniguchi Hiroki,
Hagiwara Akeo,
Yamaguchi Toshiharu,
Abe Tatsuo,
Inazawa Johji,
Yamagishi Hisakazu
Publication year - 2001
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.1522
Subject(s) - comparative genomic hybridization , colorectal cancer , primary tumor , medicine , incidence (geometry) , metastasis , biology , cancer , oncology , cancer research , gene , genetics , genome , physics , optics
Abstract Comparative genomic hybridization (CGH) was used to screen for changes in the number of DNA sequence copies in 30 primary colorectal cancers and 16 liver metastases, to identify regions that contain genes important for the development and progression of colorectal cancer. In primary colorectal cancer, we found frequent gains at 7p21 (36.7%), 7q31‐36 (30%), 8q23‐24 (43.0%), 12p (30%), 14q24‐32 (33.3%), 16p (40.0%), 20p (33.3%), 20q (63.3%) and 21q (36.3%), while loss was often noted at 18q12‐23 (36.7%). In metastatic tumors, there were significantly more gains and losses of DNA sequences than in primary tumors, with gains at 8q23‐24 (found in 62.5% of recurrences vs. 43.0% of primary tumors), 15q21‐26 (37.5% vs. 20.0%), 19p (43.8% vs. 20.0%) and 20q (81.3% vs. 63.3%) and losses at 18q12‐23 (50.0% vs. 36.7%). The pattern of genetic changes seen in metastatic tumors, with frequent gains at 8q23‐24 and 20q and loss at 18q12‐23, suggests the progression of colorectal cancer. We investigated a clinical follow‐up study for all patients examined by CGH and directed our attention to the genetic changes consisting of gains at 8q and 20q. The incidence of liver metastases was higher in patients with primary colorectal cancer with these genetic changes. Gains at 8q and 20q might be useful to identify patients at high risk for developing liver metastases. © 2001 Wiley‐Liss, Inc.