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Changes in p14 ARF do not play a primary role in human chondrosarcoma tissues
Author(s) -
Asp Julia,
Brantsing Camilla,
Benassi Maria Serena,
Inerot Sven,
Sangiorgi Luca,
Picci Piero,
Lindahl Anders
Publication year - 2001
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.1398
Subject(s) - chondrosarcoma , primary (astronomy) , medicine , biology , cancer research , pathology , physics , astronomy
The locus encoding the tumor suppressor p16 has been found to code for a second, different protein. This protein, p14 ARF , has been shown to protect p53 from degradation. Like p16, its gene is often altered in different cancers. In this study, the first unique exon, exon 1β, of p14 ARF , has been studied in 22 chondrosarcoma tissues using polymerase chain reaction, DNA sequencing and methylation‐specific polymerase chain reaction. One chondrosarcoma was found to have exon 1β homozygously deleted, but neither mutations nor methylations were found in any of the chondrosarcomas. This indicates that genetic changes of p14 ARF are a rare event in chondrosarcoma. © 2001 Wiley‐Liss, Inc.