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Characterization of chromosomal abnormalities in uroepithelial carcinomas by G‐banding, spectral karyotyping and FISH analysis
Author(s) -
Fadlelmula Imad,
Kytölä Soili,
Pan Yi,
Lui WengOnn,
Derienzo Gaetano,
Forsberg Lars,
Mandahl Nils,
Gorunova Ludmila,
Bergerheim Ulf S.R.,
Heim Sverre,
Larsson Catharina
Publication year - 2001
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.1267
Subject(s) - isochromosome , karyotype , biology , fluorescence in situ hybridization , chromosomal translocation , chromosome , cytogenetics , pathology , genetics , gene , medicine
Chromosome analysis by G‐banding, spectral karyotyping (SKY) and fluorescence in situ hybridization (FISH) was performed on 24 short‐term cultured transitional cell bladder carcinomas and 5 cell lines established from bladder carcinomas. Except for one tumor with an apparently normal chromosomal constitution, clonal chromosome abnormalities were detected in all examined cases by the combined approach. The application of SKY and FISH techniques improved the karyotypic descriptions, originally based on G‐banding only, by identifying 32 additional numerical changes, by establishing the chromosomal origin of 27 markers and 2 ring chromosomes, by redefining 53 aberrations and by detecting 15 hidden chromosomal rearrangements. No recurrent translocation, however, was detected. The most prominent karyotypic feature was thus the occurrence of deletions and losses of whole chromosome copies indicating the importance of tumor suppressor genes in transitional cell carcinoma pathogenesis. Invasive carcinomas were karyotypically more complex than were low grade superficial tumors. Specific losses of material from chromosome 9 and from chromosome arms 11p and 8p, and gains of 8q and 1q seem to be early changes appearing in superficial tumors, whereas losses from 4p and 17p and the formation of an isochromosome for 5p were associated with more aggressive tumor phenotypes. © 2001 Wiley‐Liss, Inc.