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Association of centrosomal kinase STK15/BTAK mRNA expression with chromosomal instability in human breast cancers
Author(s) -
Miyoshi Yasuo,
Iwao Kyoko,
Egawa Chiyomi,
Noguchi Shinzaburo
Publication year - 2001
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.1200
Subject(s) - biology , breast cancer , messenger rna , chromosome instability , carcinogenesis , cancer research , estrogen receptor , centrosome , cancer , microbiology and biotechnology , cell cycle , gene , chromosome , genetics
Over‐expression of a centrosomal serine/threonine kinase, STK15/BTAK, induces centrosome amplification, which results in chromosomal instability (CIN) in cell culture. In the present study, we investigated the correlation of STK15/BTAK mRNA expression with CIN and various clinicopathological factors in human breast cancer. STK15/BTAK mRNA levels were quantified by real‐time PCR, and CIN values were determined by FISH analysis of chromosomes 1, 11 and 17 using centromeric probes. STK15/BTAK mRNA levels (0.310 ± 0.413, mean ± SD, n = 47) in breast cancers were significantly ( p < 0.01) higher than those in normal breast tissues (0.044 ± 0.029, n = 9). Furthermore, breast cancers were divided into 3 groups (low, intermediate and high) according to STK15/BTAK mRNA expression levels. CIN values of the low‐expression group (27.9 ± 12.6%, n = 18) were significantly ( p < 0.01) higher than those of normal breast tissues (9.2 ± 2.6%, n = 6), and those of the high‐expression group (38.0 ± 12.7%, n = 14) were significantly ( p < 0.05) higher than those of the low‐expression group. STK15/BTAK mRNA expression showed a significant ( p < 0.05) correlation with high histological grade and negativity of estrogen and progesterone receptors. Our results demonstrate that STK15/BTAK mRNA is over‐expressed in the majority of breast cancers and its over‐expression is significantly associated with CIN, implicating STK15/BTAK in carcinogenesis through induction of CIN. STK15/BTAK mRNA levels might be useful as an indicator of poor prognosis and resistance to endocrine therapy. © 2001 Wiley‐Liss, Inc.