Premium
Identification of tumor‐associated antigens by screening phage‐displayed human cDNA libraries with sera from tumor patients
Author(s) -
Minenkova Olga,
Pucci Andrea,
Pavoni Emiliano,
De Tomassi Amedeo,
Fortugno Paola,
Gargano Nicola,
Cianfriglia Maurizio,
Barca Stefano,
De Placido Sabino,
Martignetti Angelo,
Felici Franco,
Cortese Riccardo,
Monaci Paolo
Publication year - 2003
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.11269
Subject(s) - complementary dna , cdna library , antigen , phage display , lambda phage , antibody , biology , cancer , gene , virology , immunology , bacteriophage , genetics , escherichia coli
Screening cDNA libraries from solid human tumors with sera of autologous patients (SEREX) has proven to be a powerful approach to identifying tumor antigens recognized by the humoral arm of the immune system. In many cases, application of this methodology has led to the discovery of novel tumor antigens as unknown gene products. We tried to improve the potency of the SEREX approach by combining it with phage‐display technology. We designed a new lambda vector to express protein fragments as N‐terminal fusions to the D capsid protein and generated high‐complexity cDNA libraries from human breast carcinoma cell lines and solid tumors. Screening these phage‐displayed libraries required limited amounts of sera from patients and efficiently identified several tumor antigens specifically reacting with sera from breast cancer patients. © 2003 Wiley‐Liss, Inc.