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CDKN2A region polymorphism and genetic susceptibility to melanoma in the melim swine model of familial melanoma
Author(s) -
Le Chalony Catherine,
Renard Christine,
VincentNaulleau Silvia,
Crechet Françoise,
Leplat JeanJacques,
Tricaud Yves,
Horak Vratislav,
Gruand Joseph,
Le Roy Pascale,
Frelat Gérard,
Geffrotin Claudine
Publication year - 2002
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.10871
Subject(s) - cdkn2a , biology , genetics , melanoma , haplotype , allele , genetic linkage , genetic marker , gene
Some herds of miniature swine are genetically predisposed to cutaneous melanoma. To test if swine melanoma susceptibility could be linked to the CDKN2A gene, which is involved in a proportion of 9p21‐linked human familial melanoma, we performed a genetic analysis of miniature pigs of the MeLiM strain. F 1 and backcross animals were generated by crossing 1 MeLiM boar with healthy Duroc sows. We isolated the swine CDKN2A gene and characterized a linked informative microsatellite marker, the S0644 marker. Using this marker and 2 flanking markers, we analyzed the segregation of the CDKN2A gene in a 3‐generation pedigree. Allelic association, linkage analysis and haplotype analysis of these data led to exclusion of the CDKN2A gene as a candidate for melanoma susceptibility. Nonetheless, this analysis suggests an association with the swine 1q25 chromosomal region, which is homologous to the human 9p21 region. © 2002 Wiley‐Liss, Inc.