Premium
Generation of monoclonal antibodies against Hong Kong nasopharyngeal carcinoma‐associated Epstein‐Barr virus latent membrane protein 1 (LMP1)
Author(s) -
Chan Ben Chung Lap,
To Ka Fai,
Pang Jesse Chung Sean,
Chung Yuk Fei,
Lo Kwok Wai,
Tong Joanna Hung Man,
Huang Dolly Wai Sin,
Lim Pak Leong,
Chui Yiu Loon
Publication year - 2002
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.10773
Subject(s) - monoclonal antibody , nasopharyngeal carcinoma , epstein–barr virus , virology , biology , antibody , virus , microbiology and biotechnology , immunohistochemistry , immunofluorescence , immunology , medicine , radiation therapy
A panel of monoclonal antibodies specific to Hong Kong Chinese nasopharyngeal carcinoma (NPC)‐associated Epstein‐Barr virus (EBV) latent membrane protein 1 (LMP1) variants has been generated. These monoclonal antibodies not only differentiate the Hong Kong Chinese NPC‐associated LMP1 variants from the prototype B95‐8 LMP1, derived from Caucasian infectious mononucleosis, but also differentiate the 2 highly homologous LMP1 deletion variants commonly found in Hong Kong primary NPC. The predominant deletion type variant, DV‐Asp335, is characterized by an aspartic acid at residue 335 located in the cytoplasmic C‐terminal region, whereas the other minor deletion variant, DV‐Gly335, has a glycine in the same residue position. 335D is hitherto found predominantly in LMP1 of the China 1 strain in association with NPC in the Chinese populations located in southern China and Malaysia. These antibodies, which are applicable in ELISA, immunofluorescence, immunoprecipitation, immunoblotting and immunohistochemistry on paraffin sections, are the first variant‐specific anti‐LMP1 monoclonal antibodies produced, and will be useful in investigating the functional significance of 335D in NPC. © 2002 Wiley‐Liss, Inc.