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Type III intestinal metaplasia and carcinoma express an identical carbohydrate‐epitope revealed by a novel monoclonal antibody (2D11)
Author(s) -
Kimura Akihiko,
Ohta Masataka,
Ono Kazuo,
Yamaguchi Kazuya,
Inoue Hiroshi,
Yasuda Seiji,
Nosaka Mizuho,
Tsuji Tsutomu
Publication year - 2002
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.10684
Subject(s) - intestinal metaplasia , mucin , epitope , biology , immunohistochemistry , antigen , gastric mucosa , monoclonal antibody , metaplasia , microbiology and biotechnology , stomach , pathology , antibody , medicine , biochemistry , immunology
A monoclonal antibody (2D11, IgG2b) obtained by immunizing mice with a mucin fraction of the human gastric mucosa reacted specifically to intestinal metaplasia of human gastric mucosa and fetal intestinal mucosa but not to normal adult gastric, small intestinal or colonic mucosa in immunohistochemical staining. The results of Western blotting indicated that 2D11 recognized the high molecular weight glycoprotein(s) (mucin) of the stomach. Treatment of the antigens with sodium periodate abolished their reactivity to 2D11, and digestion of the antigens with β‐galactosidase reduced their reactivity to 2D11. Digestion of the antigens with pronase had no effect, however, suggesting that 2D11 recognizes the oligosugar moiety but not the peptide moiety of the antigens. Further immunohistochemical investigation showed that the reactivity of 2D11 was restricted to the Type III intestinal metaplasia that is identified by a characteristic staining pattern with the high iron diamine‐Alcian blue stain. 2D11 also reacted in high frequency to adenocarcinomas of the stomach (66.7%), pancreas (66.7%) and gallbladder (50.0%), but in low frequency to those in lung (8.3%) and colon (11.1%). It is of interest that 2D11 reacted to very restricted regions of the gastric adenocarcinomas. All monoclonal antibodies to mucin polypeptides (MUC1, 2, 3, 5AC and 6) examined stained intestinal metaplasia and carcinomas in a different pattern from 2D11 in immunohistochemistry. These facts indicate that Type III intestinal metaplasia and carcinomas express carbohydrate chains identical to those expressed in the fetal intestinal mucosa, suggesting that both of them are closely related to fetal intestinal mucosa. © 2002 Wiley‐Liss, Inc.